Read before use
1, check data with precheck (windows version) tools
2, data from excel, copy and paste data into the input frame
3, data from txt, must tab-seperated, copy and paste data into the input frame
4, specieal and non-English characters such as #, <, >, %, (, ), α are not friendly
5, use point as decimal separator, not comma. e.g. 3.14, not 3,14 as pi

Required
PCR data:

RNA-seq data:


Optional
Figure size
figure width:
figure height:

Label PCR axis label:
RNA-seq axis label:

Fontsize
ticks fontsize:
axis label fontsize:
legend fontsize:

Colors
PCR bar color:
RNA-seq line color:

Fontfamily


PCR vs RNA-seq dual Y axis plot

Input data instructions
PCR input data contain 3 columns: the first column is name, the second column is expression, and the third column is standard deviation (in excel using: stdev(PCR value list)/sqrt(repeat number)).
RNA-seq input data contain 2 columns: the first column is name, and the second column is expression.
Examples from papers
The trend of PCR and RNA-seq is the same.
PCR input 
PCR	expression	stdev
0h	2	0.08
4h	2.5	0.12
12h	3.8	0.09
24h	4.2	0.15
RNA-seq input 
RNA-seq	expression
0h	1.2
4h	3.5
12h	5.2
24h	6.1
Output

1) How to plot?
1, Prepare data
2, Open with excel, and change into the same format as the example
3, Copy and paste data into the input frame
4, Select parameters
5, Submit and download figure files

2) Why NO figure generated?
Script need strigent input format, please read the instructions and examples carefully.

3) How to cite?
2000+ papers in (Google Scholar)
Tang D, Chen M, Huang X, Zhang G, Zeng L, Zhang G, Wu S, Wang Y. SRplot: A free online platform for data visualization and graphing. PLoS One. 2023 Nov 9;18(11):e0294236. doi: 10.1371/journal.pone.0294236. PMID: 37943830.

4) FAQs